Reinholz, M., et al., Targeted Gene Expression Profiling of Traditional Immunohistochemical Tumor Biomarkers Using Nuclease Protection Coupled with Next-Generation Sequencing.  Poster presented at: Association for Molecular Pathology (AMP), 2017 Nov; Salt Lake City, UT Download

Mian, O., et al., Molecular Profiling of Small Cell Bladder Cancer (SCBC) Reveals Gene Expression Determinants of an Aggressive Phenotype.  Poster presented at: The American Society for Radiation Oncology (ASTRO); 2017 Sept; San Diego, CA. Download

Foy JP., et al., The promising impact of molecular profiling on treatment strategies in oral cancers. J. Stomatol Oral Maxillofac Sur (2017), Download

Terry, S., et al., New insights into the role of EMT in tumor immune escape. Mol Oncol. 2017 Jul; 11(7): 824-846.

Young K., et al., Role of AKT Hyperactivation and the Potential of AKT-Targeted Therapy in Diffuse Large B-Cell Lymphoma. Am J Pathol. 2017 Apr 6; In Press. Download

Mian O., et al. Molecular profiling of small cell bladder cancer (SCBC) to reveal gene expression determinants of an aggressive phenotype. Poster presented at: The American Society of Clinical Oncology (ASCO), 2017 Jun; Chicago, IL Download

Balko J., et al. Molecular characterization of immune-related severe adverse events (irSAE). Poster session presented at: The American Society of Clinical Oncology (ASCO), 2017 Jun; Chicago, IL Download

Sunakawa Y., et al. Association of immune-related genes to neutrophil-lymphocyte ratio (NLR) with survival of cetuximab treatment for metastatic colorectal cancer (mCRC): JACCRO CC-05/06AR. Poster presented at: The American Society of Clinical Oncology (ASCO). 2017 June; Chicago, IL

Aissaoui S., HTG EdgeSeq miRNA Whole Transcriptome Assay Platform Performance Metrics Utilizing the MicroRNA (miRQC) Study Specimens. Poster presented at: The European Human Genetics Conference (ESHG). 2017 May; Copenhagen, Denmark. Download

Knudsen E., et al. Stratification of Pancreatic Ductal Adenocarcinoma: Combinatorial Genetic, Stromal, and Immunological Markers. Clin Cancer Res; 23(15); 4429-40.

Foy JP., et al. The immune microenvironment of HPV-negative oral squamous cell carcinoma from never-smokers and never-drinkers patients suggests higher clinical benefit of IDO1 and PD1/PD-L1 blockade. Annals of Oncology: 1934-1941, 2017. Download 

Reinholz, M., et al. Multi-Site Reproducibility and Analytic Accuracy of the HTG EdgeSeq Immuno-Oncology Assay. Poster session presented at: The Association for Molecular Pathology (AMP) Global, 2017 Apr; Berlin, Germany Download

Morales-Kastresana, A., et al., RNA profiling limits for nanoFACS-sorted extracellular vesicles. J Extracell Vesicles. 2017 Apr; 6(sup1): 1310414 (OT7.03). Published online 2017 May 15.

Francque S., et al. Next-Generation Sequencing (NGS) of Two Independent Cohorts Identifies Eleven Circulating mirNAs for Diagnosis of Nash and Fibrosis. Poster session presented at: The International Liver Congress (ILC), 2017 Apr; Amsterdam, Holland.  Download

Howard K., et al. A Cross Comparison of Technologies for the Detection of Immune System Related Gene Expression Signatures in Clinical FFPE Samples of Metastatic Prostate Cancer Patients.  Poster session presented at: The American Association for Cancer Research; 2017 Apr; Washington, DC. Download

Hirsch F., et al. Heterogeneity in immune biomarker expression: Detailed analysis of a case with SCLC transformation after EGFR-TKI treatment: J Thor Oncol. 2017 Apr; in press. 
Poster session presented at: The American Association for Cancer Research, 2017 Apr; Washington, DC. Download

Qi Z., et al. Reliable Gene Expression Profiling From Smaller and H&E-Stained Clinical FFPE Specimens Using HTG EdgeSeq Platform. Poster session presented at: The American Association for Cancer Research, 2017 Apr; Washington, DC Download

Balko J., et al. Advanced molecular characterization of severe autoimmune toxicities associated with checkpoint inhibitor therapies. Poster session presented at: The American Association for Cancer Research, 2017 Apr; Washington, DC Download

Danilin S. et al., Validation of our HTG EdgeSeq Platform for accurate and sensitive biomarker discovery. Poster session presented at Molecular Diagnostics Europe, 2017 Apr; 2017 Apr, Lisbon, Portugal Download

Davis B. et al. Validation of a Multiplexed Gene Signature Assay for Diagnosis of Canine Cancers from Formalin-Fixed Paraffin-Embedded Tissues. J Vet Intern Med 2017 Mar 29; doi: 10.111/jvim. 14686 [Epub ahead of print]

Sharma P., et al. Nivolumab in metastatic urothelial carcinoma after platinum therapy (CheckMate 275): a multicentre, single-arm, phase 2 trial. Lancet. 2017 Jan 25; [Epub ahead of print].

Harpole D., et al. Creation and Early Validation of Prognostic miRNA Signatures for Squamous Cell Lung Carcinoma by the SPECS Lung Consortium. J Thorac Oncol. 2017 Jan; 12(1): S612-S613.




Margolles-Clark E., Ruiz P. P048 Gene Expression Profiling of Transplant Formalin-Fixed Paraffin-Embedded Biopsies: Comparison of A Custom Taqman® Low Density Array and Quantitative Nuclease-Protection Assay. Human Immunology. 2016 Sept;77(Supplement):74.  Download

Lizarraga D., et al. miRNAs differentially expressed by next-generation sequencing in cord blood buffy coat samples of boys and girls. Epigenomics. 2016 Dec; 8(12):1619-1635.

Lo B., et al. Combining Automated Tissue Dissection with Extraction Free Nucleic Acid Analysis. J Mol Diag. 2016, 18: 1048.  Download

Qi Z., et al. Evaluation and selection of a non-PCR based technology for improved gene expression profiling from clinical formalin-fixed, paraffin-embedded samples. Bioanalysis. 2016 Oct 7. [Epub ahead of print]

Girard L., et al. An Expression Signature as an Aid to the Histologic Classification of Non-Small Cell Lung Cancer. Clin Cancer Res. 2016 Oct 1;22(19):4880-4889.

Triozzi P.L., et al. Association of tumor and plasma microRNA expression with tumor monosomy-3 in patients with uveal melanoma. Clin Epigenetics. 2016 Jul 22;8:80.

DeLoach J., et al. Repeatability and reproducibility of an automated, multiplex p450 enzyme gene expression assay. Drug Metab Rev. 2016 Jul; 48(Suppl 1):177.

LeCluyse E., et al. A multiplex approach to investigate drug-induced changes in p450 enzyme gene expression. Drug Metab Rev. 2016 Jul; 48(Suppl 1):22.   Download (PDF 616KB)

Lipchik C, et al. FFPE Tissue and the HTG Oncology Biomarker Panel: a Low-Input, Extraction-Free Gene Expression Pilot Study. Poster presented at:  The Mid-Atlantic Directors and Staff of Scientific Cores; 2016; Pittsburgh, PA.  Download (PDF 2.93MB)   

Reinholz M, et al. Next-Generation Sequencing from DLBCL Classification. J Clin Oncol. 2016;34(Suppl):abstr 11559. Download (PDF 740KB)

Sunakawa, Y., et al. Association of gene signature to identify molecular subtypes with clinical outcomes of 1st-line cetuximab (cet) treatment for metastatic colorectal cancer (mCRC). J Clin Oncol. 2016;34(Suppl):abstr 3592. 

Sunakawa, Y., et al. Immune-related genes to predict clinical outcome of cetuximab (cet) treatment for metastatic colorectal cancer (mCRC): Immuno-Oncology assay research. J Clin Oncol. 2016;34(Suppl):abstr. 11591.

Reinholz M., et al. Verification of miRNA Expression Using Nuclease Protection and Targeted Next-Generation Sequencing. Poster session presented at The European Society of Human Genetics; 2016; Barcelona, Spain. Download (PDF 2.07MB)

Wang, S., et al. Hsa-miR-24-3p increases nasopharyngeal carcinoma radiosensitivity by targeting both the 3′UTR and 5′UTR of Jab1/CSN5. Oncogene. 2016 Nov 24; 35(47):6096–6108.

Hollander, Z., et al. Biomarker Monitoring for Absence of Acute Allograft Rejection. J Heart Lung Transplant. 2016 Apr; 35(4):S33.

Reinholz M., et al. Gene Expression Measurement of Immuno-Oncology Targets in a Single FFPE Section Using a Novel Targeted Sequencing Assay. Cancer Res. July 15 2016;76(14 Suppl):abstr 2237. Download (PDF 1.52MB)

Reinholz M., et al. NGS-based measurement of gene expression of 2560 oncology-related biomarkers in formalin-fixed, paraffin-embedded (FFPE) tissues. Cancer Res. 2016;76(14 Suppl):abstr 1383. 

Reinholz M., et al. The HTG EdgeSeq Oncology Biomarker Panel enables NGS-based gene expression profiling from small samples Poster session presented  at:  Association of Biomedical Research Cores; 2006 Feb  20-23; Fort  Lauderdale, FL  Download (PDF 1.13MB) 

Botros I., et al. Unique Endogenous Controls for Extraction-Free Targeted RNA Sequencing Assays. Poster session presented at: Advances in Genome Biology and Technology; 2016 Feb 10-13; Orlando, FL. Download (PDF 2.2MB)

Semrad, T., et al. Phase II study of dovitinib in patients progressing on anti-vascular endothelial growth factor therapy. J Clin Oncol. 2016:34(Suppl 4S):A616. Download (PDF 847KB)

Sunakawa, Y., et al. Alteration of ADCC-related genes as a novel predictor of efficacy of cetuximab (cet)-based chemotherapy in patients (pts) with metastic colorectal cancer (mCRC). J Clin Oncol. 2016 Jul;34(Suppl 4S):A589. Download (PDF 463KB)

DeYoung, M. P., et al., Inhibition of FGF/FGFR autocrine signaling in mesothelioma with FGF ligan Trap, FP-10389/GSK3052230; Oncotarget 2016 June 28; 7(26): 29861-39871.



Xu-Monette, Z.Y., et al. Diffuse Large B-Cell Lymphoma Cell of Origin Determination from Formalin-Fixed Paraffin-Embedded Tissues. Blood 2015 126:5052.

Barczak A.J., et al.  Reproducibility and Bias of miRNA Profiling with the HTG EdgeSeq Targeted Capture and Sequencing Assay. Poster session presented at: The External RNA Control Consortium (ERCC) 2015 meeting.

Chen L.Q., et al. Assessment of carbonic anhydrase IX expression and extracellular ph in B-cell lymphoma cell line models. Leuk Lymphoma. 2015 May; 56(5):1432–1439.

Baker, A.F., et al. Evaluation of a Hypoxia Regulated Gene Panel In Ovarian Cancer. Cancer Microenviron. 2015 Apr;8(1):45-56. Baker,  Download (PDF 864K)

Reinholz. M., et al. HTG EdgeSeq Oncology Biomarker Panel (OBP), an NGS-based Measurement of Gene Expression of 2,532 Oncology-Related genes in FFPE tissue in different sample types. Poster session presented at: 8th GTCBio Oncology Biomarkers Conference; 2015, San Diego, CA. Download (PDF 1.09MB)

Young, K., et. al, Age cutoff for Epstein-Barr virus-positive diffuse large B-cell lymphoma-is it necessary? Oncotarget 2015 June 10; 6(16): 13933-13945.

Liang J., et al. LEF1 targeting EMT in prostate cancer invasion is mediated by miR-181a. Am J Cancer Res. 2015 Feb 15;5(3):1124-1132.

Maddula K., et al. Targeted RNA Sequencing for simultaneous expression profiling and detection of gene rearrangements in FFPE biopsies. Poster session presented at: Advances in Genome Biology and Technology; 2015; Marco Island, FL. Maddula K, et al. Download (PDF 1.15MB)

Ono, S., et. al., Circulating microRNA Biomarkers as Liquid biopsy for Cancer Patients: Pros and Cons of Current Assays. J Clin Med. 2015 Oct; 4(10): 1890-1907.

Schwartz, M, et al. Repeatability and reproducibility of an automated, multiplex p450 enzyme gene expression assay. Poster presented at 2015 ISSX; 2015 Oct 18 - 22. Download (PDF 1.2MB)



Thompson D., et al. Automated high fidelity miRNA expression profiling using nuclease protection coupled with next generation sequencing. Poster session presented at: 64th annual meeting of the American Society of Human Genetics; 2014 October 18-22; San Diego, CA  Download

Rowley, A. H., et al. A Study of Cardiovascular miRNA Biomarkers for Kawasaki Disease.  Pediatr Infect Dis J. 2014 Dec; 33(12): 1296-1299.

Barr P.M., et al. Phase 2 study of imexon, a prooxidant molecule, in relapsed and refractory B-cell non-Hodgkin lymphoma. Blood. Aug 2014 Vol 124:8.

Ren Q., et al. Epithelial and stromal expression of miRNAs during prostate cancer progression. Am J Transl Res. 2014 Jul 18;6(4):329-39.

Thompson D., et al. Automated high fidelity miRNA expression profiling using nuclease protection coupled with next generation sequencing. Poster session presented at: Merck Technology Symposium; 2014 May; Long Branch, NJ. Download (PDF 1.49MB)

Maddula K., et al. FGFR and FGF ligand overexpression in lung cancer: Implications for targeted therapy. J Clin Oncol. 2014;32(Suppl 5):abstr 11080. Download

Shell S., et al. Correlation of multiplex measurement of mRNA expression from FFPE tissues with protein expression. J Clin Oncol. 32, 2014;32(Suppl):abstr e22141.FFPE tissues with protein expression. J Clin Oncol. 32, 2014;32(Suppl):abstr e22141.

Maddula K., et al. RNA expression based screening of ALK Gene Fusion from NSCLC FFPE samples. Cancer Res. 2014;74(19 Suppl):abstr 1904.

Wang E., et al. Non-small cell lung cancer histological sub-typing by gene expression analysis from FFPE tissue. Cancer Res. 2014;74(19 Suppl):abstr 1877.

Hollander Z., et al. Blood test to Monitor for the Absence of Acute Cardiac Rejection: From Discovery to Clinical Implementation. J Heart Lung Transplant. 2014 Apr; 32(Suppl 4).

McPherson C., et al. In Vivo Molecular Markers for Pro-inflammatory Cytokine M1 Stage and Resident Microglia in Trimethyltin-Induced Hippocampal Injury. Neurotox Res. 2014 Jan 25(1):45-56.

Whitman S, et al. A multiplex approach to investigate drug-induced changes in p450 enzyme gene expression.Poster presented at 2014 ISSX; 2014 Oct 19 - 23. Download (PDF 2.5MB)



Liu Z., et al. miR-106a Represses the Rb Tumor Suppressor p 130 to Regulate Cellular Proliferation and Differentiation in High-Grade Serous Ovarian Carcinoma. Mol Cancer Res. 2013 Nov 1;11(11):1314-1325.

Rounseville M., et al. Development of an automated RNA-based nuclease protection assay for the detection of expressed EML-ALK fusions, EGFR and KRAS mutations in NSCLC. J Clin Oncol. 2013;31(Suppl); abstr e22185.

Seligmann B., et al. miRNA Biomarker Identification Using a Sequencing-Based Nuclease Protection Assay (NPSeq™). J Biomol Tech. 2013 May;24(Suppl). Download (PDF 694KB)

Thompson D., et al. Targeted gene expression sequencing from FFPE: NPSeq™. Cancer Res. 2013;73(8 Suppl):abstr 4146. Download 

Rounseville M., et al. Comparison of qNPA vs qPCR on FFPE tissue. Cancer Res. 2013;73(8 Suppl):abstr 4133. Download (PDF 733KB)

Yang D., et al. Array-based Quantitative Nuclease Protection Assay Can Reproducibly Identify Prognostic mRNA Biomarkers in Archival Mantle Cell Lymphoma Specimens. Mod Pathol. 2013 Feb;26(S2):368A. Download (PDF 1.1MB)

Seligmann B., et al. miRNA Biomarker Identification Using a Sequencing-Based Nuclease Protection Assay (NPSeq): Building on Edge Automation of qNPA for RNA Measurement. J Biomol Tech. 2013 May;24(Suppl):S34-S35.

Chandler K., et al. Investigation of a Redox-Sensitive Predictive Model of Mouse Embryonic Stem Cell Differentiation via Quantitative Nuclease Protection Assays and Gluthione Redox Status. Poster session presented at: 52nd Annual Society of Toxicology Meeting; 2013 Mar 10-14, San Antonio, TX.

Rounseville M., et al. Automated Measurement of Gene Expression from FFPE: qNPA vs. qPCR. Poster session presented at: Molecular Med Tri-Con; 2013 Feb 11-15; San Francisco, CA   Download (PDF 849KB)

Barr P.M., et al. Redox Associated Gene Expression Predicts for Responses to The Pro-Oxidant Molecule Imexon in Relapsed and Refractory B-Cell Non-Hodgkin Lymphoma: Results of a Multi-Center Phase II Study. Blood. 2013;122(21):89.

Modur  V, et al. Evidence-based laboratory medicine in oncology drug development: From biomarker to diagnostics. Clin Chem. 2013 Jan;59(1):102-9.

Hunter E, et al. Investigation of a Redox-Sensitive Predictive Model of Mouse Embryonic Stem Cell Differentiation via Quantitative Nuclease Protection Assays and Glutathione Redox Status. Poster presented at the 52nd Annual Society of Toxicology Meeting; 2013 Mar 10-14 (abstr 1205).

Brengues M, et al. Biodosimetry on Small Blood Volume using Gene Expression Assay. Health Phys. Author manuscript; PMC 2013 June 10.



Erickson H.S. Measuring molecular biomarkers in epidemiologic studies: laboratory techniques and biospecimen considerations. Stat Med. 2012 Sep 28;31(22):2400-13.

Gerson K., et al. Effects of B4 integrin expression on microRNA patterns in breast cancer. Biol Open. 2012 Jul 15; 1(7):658-666.

Guerau-de-Arellano M., et al. miRNA profiling for biomarker discover in multiple sclerosis: From microarray to deep sequencing. J Neuroimmunol. 2012 Jul 15; 248(1-2):32-39.

Bakir F., et al. Discovery and Structure-Activity Relationship Studies of Indole Derivatives as Liver X Receptor (LXR) Agonists. Bioorg Med Chem Lett. 2007 Jun 15;17(12): 3473–3479.



Evangelist M., et al. Quantitative Nuclease Protection Assay (qNPA) for Gene Expression Analysis on Brest Cancer Core Biopsies. J Clin Oncol. 2011 Sep 20;29(Suppl 27);abstr 49.

Bourzac K., et al. A High-Density Quantitative Nuclease Protection Microarray Platform For High Throughput Analysis of Gene Expression. J Biotechnol. 2011 Jun 10;154(1):68-75.

Rimsza L., et al. Accurate Classification of Diffuse Large B cell Lymphoma into Germinal Center and Activated B cell Subtypes Using a Nuclease Protection Assay on Paraffin Embedded Tissues. Clin Cancer Res. 2011 Jun 1:17(11)

Bellmunt, J. Identification of ALK gene alterations in urothelial carcinoma (UC). J Clin Oncol. 2011 Sep 20;29(Suppl 27);abstr 4568.

Rimsza L, et al. A Strategy for Full Interrogation of Prognostic Gene Expression Patterns: Exploring the Biology of Diffuse Large B Cell Lymphoma. PLoS One. 2011;6(8):e22267.



Yang D., et al. Identification of Potential Prognostic Biomarkers in Mantle Cell Lymphoma by Array-Based Quantitative Nuclease Protection Assay. Blood. 2010 Nov 19;116: abstr 2007.

Rotroff D., et al. Xenobiotic-metabolizing enzyme and transporter gene expression in primary cultures of human hepatocytes modulated by ToxCast chemicals. J Toxicol Environ Health B Crit Rev. 2010 Feb;13(2-4):329-346.

Brengues M., et al. Biodosimetry on Small Blood Volume using Gene Expression Assay. Health Phys. 2010 Feb;98(2):179-185.



Wilkinson S., et al. A Comparison of Gene Expression Pattern in Major Histocompatibility Class II-Low Diffuse Large B-Cell Lymphoma with Plasmablastic Lymphoma. Blood. 2009;114:abstr 1941.

Pechold S, et al. Transcriptional Analysis of Intracytoplasmically Stained, FACS-Purified Cells by High Throughput, Quantitative Nuclease Protection. Nat Biotechnol. 2009 Nov;27(11):1038-1042.

Coker P., et al. Precise Multiplexed Measurement of Gene Expression: Quantitative Nuclease Protection Assay (qNPA™). Proceedings of the 35th Annual Meeting of the Plant Growth Regulation Society of America.

Trifonova R., et al. Polyanionic Microbicides Modify Toll-Like Receptor-Mediated Cervicovaginal Immune Responses. Antimicrob Agents Chemother. 2009 Apr;53(4):1490-1500.

Altar C, et al. Target Identification for CNS diseases by transcriptional profiling. Neuropsychopharmacology. 2009 Jan;34(1);18-54.



Altar C., et al. Insulin, IGF-1, and Muscarinic Agonists Modulate Schizophrenia-Associated Genes in Human Neuroblastoma Cells. Biol Psychiatry. 2008 Dec 15;64(12);1077-1087.

Rimsza L., et al. Gene Expression Predicts Overall Survival in Paraffin Embedded Tissues of Diffuse Large B Cell Lymphoma Treated with R-CHOP. Blood. 2008 Oct 15;112 (8):3425-3433.

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