Masthead - miRNA WTA
HTG EdgeSeq miRNA Whole Transcriptome Assay

HTG EdgeSeq miRNA Whole Transcriptome Assay

The HTG EdgeSeq miRNA Whole Transcriptome Assay (miRNA WTA) enables users to measure the expression of 2,083 human miRNA transcripts using next-generation sequencing (NGS). The HTG EdgeSeq chemistry is compatible with fixed tissue samples, biofluids, and cell lines. The miRNA WTA utilizes our automated HTG EdgeSeq system coupled with the sensitivity and dynamic range of NGS-based detection, and can be performed in your lab using the HTG EdgeSeq system, from our VERI/O lab as a service, or at one of the Qualified Service Provider (QSP) sites.

For Research Use Only. Not for Use in Diagnostic Procedures.

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Features and Benefits

  • Simultaneous, quantitative detection of 2,083 human miRNA transcripts
  • Profile circulating miRNA: obtain miRNA profiles from 15 µl of plasma or serum
  • Extraction-free: reduce extraction-associated data bias and sample loss
  • Gain insights faster: results in as little as 36 hours
  • Simplified data analysis: raw numerical data output provided in Excel format

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Sample Requirements

Sample Type HTG EdgeSeq miRNA Whole Transcriptome Assay

Larger sample input amounts must be diluted. Please consult the assay package insert and HTG EdgeSeq System User Manual for more details. 

FFPE TissueOne 5 µm section
Extracted RNA from Frozen10 - 35 ng
Extracted RNA from FFPE35 ng / 35 µl
PAXgene500 µl
Plasma / Serum≥15 µl
Cells≥3,000 cells

Performance

Pearson correlations of >0.95 are routinely obtained between technical replicates of plasma (left chart) and FFPE (right chart). 

Figure 1

Figure 2

Applications

miR-205 is a specific marker for squamous cell carcinoma in lung cancer (Lebanony, et al., 2009). The
HTG EdgeSeq miRNA Whole Transcriptome Assay detects miR-205, differentiating adeno and squamous cell lung carcinomas. A miRNA reported to be expressed in both lung cancer subtypes, let7a-5p, is displayed for reference.

miR-205 expression
let7a-5p expression

Ordering Information

When placing an order, please specify the catalog number. The HTG EdgeSeq miRNA Whole Transcriptome Asssay is compatible with Illumina sequencers and Thermo Fisher Ion Torrent sequencers.

Kit Configurations for use with Illumina Sequencers

916-001-208 HTG EdgeSeq miRNA WTA (2x8)
916-001-008 HTG EdgeSeq miRNA WTA (4x8)
916-001-224 HTG EdgeSeq miRNA WTA (1x24)
916-001-024 HTG EdgeSeq miRNA WTA (4x24)
916-001-096 HTG EdgeSeq miRNA WTA (1x96)

Kit Configurations for use with Thermo Fisher Ion Torrent Sequencers

916-001-308 HTG EdgeSeq miRNA WTA (2x8)
916-001-108 HTG EdgeSeq miRNA WTA (4x8)
916-001-324 HTG EdgeSeq miRNA WTA (1x24)
916-001-124 HTG EdgeSeq miRNA WTA (4x24)

For Research Use Only. Not for Use in Diagnostic Procedures.

Request A Quote

Resources and Publications

Learn more about the HTG EdgeSeq miRNA Whole Transcriptome Assay

Resources

Biopharma and HTG Molecular Diagnostics Brochure
Download pdf 678KB

HTG EdgeSeq miRNA Whole Transcriptome Assay Cross-Species Gene List
Download pdf 746KB

HTG EdgeSeq miRNA Whole Transcriptome Assay Product Sheet
Download pdf 532KB

Publications


Download pdf 1.1MB

MicroRNAs in CSF as prodromal biomarkers for Huntington disease in the PREDICT-HD study
Download pdf 547KB

Creation and Early Validation of Prognostic miRNA Signatures for Squamous Cell Lung Carcinoma by the SPECS Lung Consortium
View External Link

HTGEdgeSeq miRNA Whole Transcriptome Assay Platform Performance Metrics Utilizing the MicroRNA (miRQC) Study Specimens
Download pdf 1.1MB

Next-Generation Sequencing (NGS) of Two Independent Cohorts Identifies Eleven Circulating miRNAs for Diagnosis of Nash and Fibrosis
Download pdf 934KB

Validation of our HTG EdgeSeq Platform for accurate and sensitive biomarker discovery
Download pdf 3.2MB

Evaluation of a novel microRNA Whole Transcriptome Assay
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Next-Generation Sequencing (NGS) of Two Independent Cohorts Identifies Eleven Circulating mirNAs for Diagnosis of Nash and Fibrosis
Download pdf 934KB

Verification of miRNA Expression using Nuclease Protection and Targeted Next-Generation Sequencing (NGS)
Download pdf 2.1MB

miRNAs differentially expressed by next-generation sequencing in cord blood buffy coat samples of boys and girls
View External Link

Association of tumor and plasma microRNA expression with tumor monosomy-3 in patients with uveal melanoma
View External Link

Combining Automated Tissue Dissection with Extraction Free Nucleic Acid Analysis
View External Link


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For further information on the HTG EdgeSeq miRNA Whole Transcriptome Assay, please fill out the registration form below or call us at (877) 507-3259.

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Page last updated November 06, 2017