HTG EdgeSeq miRNA Whole Transcriptome Assay
The HTG EdgeSeq miRNA Whole Transcriptome Assay (miRNA WTA) enables users to measure the expression of 2,083 human miRNA transcripts using next-generation sequencing (NGS). The HTG EdgeSeq chemistry is compatible with fixed tissue samples, biofluids, and cell lines. The miRNA WTA utilizes our automated HTG EdgeSeq system coupled with the sensitivity and dynamic range of NGS-based detection and can be performed in your lab using the HTG EdgeSeq system, from our VERI/O lab as a service, or at one of the Qualified Service Provider (QSP) sites.
For Research Use Only. Not for Use in Diagnostic Procedures.
Features and Benefits
- Simultaneous, quantitative detection of 2,083 human miRNA transcripts
- Profile circulating miRNA: obtain miRNA profiles from 15 µl of plasma or serum
- Extraction-free: reduce extraction-associated data bias and sample loss
- Gain insights faster: results in as little as 36 hours
- Simplified data analysis: raw numerical data output provided in Excel format
Sample Requirements
| Sample Type | Recommended Sample Input |
|---|---|
Larger sample input amounts must be diluted. Please consult the assay package insert and HTG EdgeSeq System User Manual for more details. |
|
| FFPE Tissue | One 5 µm section |
| Extracted RNA from Frozen | 10 - 35 ng |
| PAXgene | 500 µl |
| Extracted RNA from FFPE | 35 ng |
| Plasma / Serum | ≥15 µl |
| Cells | ≥3,000 cells |
| Extracted RNA | 10 - 35 ng |
Performance
Pearson correlations of >0.95 are routinely obtained between technical replicates of plasma (left chart) and FFPE (right chart).
Figure 1
Figure 2
Applications
miR-205 is a specific marker for squamous cell carcinoma in lung cancer (Lebanony, et al., 2009). The
HTG EdgeSeq miRNA Whole Transcriptome Assay detects miR-205, differentiating adeno and squamous cell lung carcinomas. A miRNA reported to be expressed in both lung cancer subtypes, let7a-5p, is displayed for reference.
miR-205 expression
let7a-5p expression
Ordering Information
When placing an order, please specify the catalog number. The HTG EdgeSeq miRNA Whole Transcriptome Asssay is compatible with Illumina sequencers and Thermo Fisher Ion Torrent sequencers.
Kit Configurations for use with Illumina Sequencers
916-001-208 HTG EdgeSeq miRNA WTA (2x8)
916-001-008 HTG EdgeSeq miRNA WTA (4x8)
916-001-224 HTG EdgeSeq miRNA WTA (1x24)
916-001-024 HTG EdgeSeq miRNA WTA (4x24)
916-001-096 HTG EdgeSeq miRNA WTA (1x96)
Kit Configurations for use with Thermo Fisher Scientific Ion Torrent Sequencers
916-001-308 HTG EdgeSeq miRNA WTA (2x8)
916-001-108 HTG EdgeSeq miRNA WTA (4x8)
916-001-324 HTG EdgeSeq miRNA WTA (1x24)
916-001-124 HTG EdgeSeq miRNA WTA (4x24)
916-001-196 HTG EdgeSeq miRNA WTA (1X96)
For Research Use Only. Not for Use in Diagnostic Procedures.
Resources and Publications
Learn more about the HTG EdgeSeq miRNA Whole Transcriptome Assay
MicroRNAs in CSF as prodromal biomarkers for Huntington’s disease
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Evaluation of a novel microRNA Whole Transcriptome Assay
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HTG EdgeSeq miRNA Whole Transcriptome Assay identifies miRNA expression signatures in biofluids aiding in forensic identification
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HTG EdgeSeq miRNA Whole Transcriptome Assay Cross-Species Gene List
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HTG EdgeSeq miRNA Whole Transcriptome Assay Product Sheet
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Publications
TP53 gain-of-function mutation modulates the immunosuppressive microenvironment in non-HPV associated oral squamous cell carcinoma
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The microRNA Cargo of Human Vaginal Extracellular Vesicles Differentiates Parasitic and Pathobiont Infections from Colonization by Homeostatic Bacteria
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ASH 2022: 4265: Durable Responses from Acalabrutinib in Combination with Rituximab, Cyclophosphamide, Doxorubicin, Vincristine and Prednisolone (R-CHOP) As First Line Therapy for Patients with Diffuse Large B-Cell Lymphoma (DLBCL): The Accept Phase Ib/II Single Arm Study
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What Happens In Utero Does Not Stay In Utero: a Review of Evidence for Prenatal Epigenetic Programming by Per- and Polyfluoroalkyl Substances (PFAS) in Infants, Children, and Adolescents
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Host miRNAs as biomarkers of SARS-CoV-2 infection: a critical review
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Plasma circulating microRNAs associated with obesity, body fat distribution, and fat mass: the Rotterdam Study
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Plasma microRNA signature of alcohol consumption: the Rotterdam Study
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MicroRNA-375 repression of Kruppel-like factor 5 improves angiogenesis in diabetic critical limb ischemia
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Genome-Wide Profiling of Circulatory microRNAs Associated with Cognition and Dementia
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A Cross-Comparison of High-Throughput Platforms for Circulating MicroRNA Quantification, Agreement in Risk Classification, and Biomarker Discovery in Non-Small Cell Lung Cancer
Small RNA-Sequencing for Analysis of Circulating miRNAs: Benchmark Study
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miR-106b-25 Dysregulation in Complex Regional Pain Syndrome Contributes to T Cell Dysfunction
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Lung Fibrosis Is Improved by Extracellular Vesicles from IFNγ-Primed Mesenchymal Stromal Cells in Murine Systemic Sclerosis
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Wildfires and extracellular vesicles: Exosomal MicroRNAs as mediators of cross-tissue cardiopulmonary responses to biomass smoke
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Establishment of a novel multi-omic biomarker panel in cyst fluid and blood for stratifying patient risk of pancreatic cancer
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Extracellular miR-6723-5p could serve as a biomarker of limbal epithelial stem/progenitor cell population
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T-cell prolymphocytic leukemia is associated with deregulation of oncogenic microRNAs on transcriptional and epigenetic level
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Placental genomics mediates genetic associations with complex health traits and disease
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Cellular miR-150-5p may have a crucial role to play in the biology of SARS-CoV-2 infection by regulating nsp10 gene
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Cell-free plasma microRNAs that identify patients with glioblastoma
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Blood-based multi-omic signatures of blood-brain barrier impairment and CSF-defined Alzheimer disease in older adults
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Kikuchi-Fujimoto disease is mediated by an aberrant type I interferon response
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Increased serum miR-193a-5p during non-alcoholic fatty liver disease progression: diagnostic and mechanistic relevance
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Multi-omic profiling of patient pancreatic cyst fluid for the identification of a novel biomarker panel of patient cancer risk
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Circulating MicroRNAs in Relation to Esophageal Adenocarcinoma Diagnosis and Survival
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Circulating microRNA Breast Cancer Biomarker Detection in Patient Sera with Surface Plasmon Resonance Imaging Biosensor
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Biomarkers and Lung Cancer Early Detection: State of the Art
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Comparing the Predictivity of Human Placental gene, microRNA, and CpG Methylation Signatures in Relation to Perinatal Outcomes
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Downregulation of the Ubiquitin-E3 Ligase RNF123 Promotes Upregulation of the NF-κB1 Target SerpinE1 in Aggressive Glioblastoma Tumors
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Kinetics Analysis of Circulating microRNAs Unveils Markers of Failed Myocardial Reperfusion.
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Epigenetic-sensitive Pathways in Personalized Therapy of Major Cardiovascular Diseases
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MicroRNA-based risk scoring system to identify early-stage oral squamous cell carcinoma patients at high-risk for cancer-specific mortality
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Synovial-Fluid miRNA signature for Diagnosis of Juvenile Idiopathic Arthritis
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Circulating microRNAs in Fabry Disease
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Circulating miRNA Profiling of Women at High Risk for Ovarian Cancer
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Hepatocyte-Derived Exosomes Promote Liver Immune Tolerance: Possible Implications for Idiosyncratic Drug-Induced Liver Injury
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MicroRNAs: Key Regulators to Understand Osteoclast Differentiation?
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Detection of a MicroRNA molecular signature of ultraviolet radiation in the superficial regions of melanocytic nevi on sun-exposed skin
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MicroRNAs in CSF as prodromal biomarkers for Huntington disease in the PREDICT-HD study
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Developmental profiling of microRNAs in the human embryonic inner ear.
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Creation and Early Validation of Prognostic miRNA Signatures for Squamous Cell Lung Carcinoma by the SPECS Lung Consortium
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Circulating miRNAS profile and risk of end-stage renal disease in type 1 diabetes
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HTG EdgeSeq miRNA Whole Transcriptome Assay Platform Performance Metrics Utilizing the MicroRNA (miRQC) Study Specimens
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Next-Generation Sequencing (NGS) of Two Independent Cohorts Identifies Eleven Circulating miRNAs for Diagnosis of Nash and Fibrosis
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Validation of our HTG EdgeSeq Platform for accurate and sensitive biomarker discovery
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Next-Generation Sequencing (NGS) of Two Independent Cohorts Identifies Eleven Circulating mirNAs for Diagnosis of Nash and Fibrosis
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Verification of miRNA Expression using Nuclease Protection and Targeted Next-Generation Sequencing (NGS)
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miRNAs differentially expressed by next-generation sequencing in cord blood buffy coat samples of boys and girls
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Association of tumor and plasma microRNA expression with tumor monosomy-3 in patients with uveal melanoma
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Learn More
For further information on the HTG EdgeSeq miRNA Whole Transcriptome Assay, please fill out the registration form below or call us at (877) 507-3259.
Page last updated June 29, 2022