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Technology

Rethink Possible with the HTG EdgeSeq Workflow and Chemistry

Our technology has a number of key differentiators, including multiplexing from hundreds to thousands of biomarkers in a variety of samples, including formalin-fixed, paraffin-embedded (FFPE) tissue; very low sample input requirements; simple, extraction-free sample preparation; automated workflow with sample-to-result turnaround times in 2 days; and simplified data output. In addition, our HTG EdgeSeq technology generates a molecular profiling library for detection using next‑generation sequencing (NGS).  NGS provides improved sensitivity and dynamic range for our HTG EdgeSeq assays. 

HTG EdgeSeq Chemistry

The HTG EdgeSeq chemistry delivers a simple, sample and library preparation method for sequencing-based quantitative assays. The schematic below provides an overview of the chemistry steps from sample to library prep with addition of tags (molecular barcodes) and sequencing adapters. The pooled libraries may be sequenced using most NGS platforms.

HTG EdgeSeq Workflow

Our solutions enable fast, reliable, reproducible, and quantitative analysis of hundreds or thousands of targeted RNAs from a single assay in as little as 36 hours with reduced hands-on time.  HTG ‘s extraction-free chemistry significantly reduces sample input requirements and preserves valuable specimens.  The HTG EdgeSeq system simplifies NGS library preparation workflow and eliminates time consuming and technically challenging steps like cDNA synthesis, size selection, removal of rRNA, mRNA end repair, and adapter ligation resulting in more reproducible results.  HTG’s intuitive graphic user interface simplifies system setup and data analysis.

HTG EdgeSeq Assays

HTG offers a variety of profiling assays for both research and diagnostic uses.

Research Use Only (RUO) Assays

Page last updated January 03, 2018

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