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Evaluation of a novel microRNA Whole Transcriptome Assay

MicroRNAs (miRNAs) are short, ~22nt RNA sequences that are believed to modulate gene transcription and downstream cell behavior. There are several platforms used for quantitation of low-abundance miRNA targets found in circulating human bodily fluids. These were evaluated in a miRNA quality control (miRQC) study (Nature Methods 11, 809-815, 2014), which compared 12 commercially available platforms for analysis of miRNA expression to identify their strengths and weaknesses, and help guide informed selection of a quantitative microRNA gene expression platform for particular study goals.

In this webcast, the performance of the HTG EdgeSeq miRNA Whole Transcriptome Assay (WTA) is evaluated using the samples and methods from the miRQC study. This is a new technology that enables users to measure the expression of 2,083 human miRNA transcripts using next-generation sequencing.

In this webcast you will

  • Learn about the objectives and strategy for genomic platform comparison studies described by the MicroArray Quality Control (MAQC) consortium.
  • Understand performance metrics used in platform comparison studies.
  • Discover key analytic outcomes through a case study utilizing the HTG EdgeSeq miRNA Whole Transcriptome Assay.


Bonnie LaFleur

Speaker Biography

Dr. LaFleur is a quantitative scientist with extensive experience in scientific research.  She has developed regulatory strategies for immuno-oncology companion diagnostic FDA-approved products and has been a key contributor to multiple in vitro diagnostic device submissions.  Dr. LaFleur has published over 90 peer-reviewed publications focusing on genomic biomarkers

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