HTG EdgeSeq Lymphoma Panel

The HTG EdgeSeq Lymphoma Panel pairs the sensitivity and dynamic range of next generation sequencing with HTG EdgeSeq extraction-free chemistry. Unlike traditional, single-analyte methods like immunohistochemistry (IHC) and RT-PCR, the HTG EdgeSeq Lymphoma Panel enables researchers to consolidate the measurement of many biomarkers in a single assay.

The HTG EdgeSeq Lymphoma Panel measures expression of 92 genes frequently assessed in lymphomas, including 22 common non-Hodgkins lymphoma (NHL) B-cell lymphoma markers from just a single 5 micron thick formalin-fixed, paraffin embedded (FFPE) section. This enables detailed molecular profiles of lymphomas using a single FFPE slide.

 

Profile 92 lymphoma-associated genes

Complement DNA variant studies with targeted RNA profiling powered by NGS

Use a single 5 µm FFPE tissue section

Conserve precious FFPE tissue samples

 

Automated on the HTG EdgeSeq system

Additional Features & Benefits:

  • Extraction-free chemistry
    Eliminate biases introduced by RNA extraction
  • IHC markers for lymphoma sub-typing
    Recreate multiple classification schemes from a single tissue section

 

Sample Input & Compatibility*

Sample Type Recommendation
FFPE Tissue A single 5 µm FFPE tissue section

*Use and performance of other sample types must be verified by the customer.

Gene List



Drug Targets
AKT1 CD19 CD22 CD274
CD276 CD37 CD70 CTLA4
EZH2 FCER2 LAG3 MS4A1
PDCD1 PDCD1LG2 STAT3 TNFRSF8 (CD30)
Lymphoma Markers
BAG5 BAK1 BCL2 BCL6
BTK CASP7 CCND1 CCND2
CCT7 CD15 (FUTY) CD3D CD4
CD47 CD5 CD68 CD79A
CD86 CD8A CDK16 CDKN1B
ENTPD1 FCGR3A FOXP1 FUT8
GRB2 HLA-DRA IL13 IL16
IL4I1 IRF4 ITPKB LMO2
LRMP MAL MAP2K7 MAP3K13
MKI67 MME MYBL1 MYC
NCAM1 NCOA1 NF2 PAICS
PAX5 PIM1 POU2AF1 POU2F2
PTPRC REL RRM2 SERPINA9
SMS SPI1 SPN SREBF1
STAT6 SUV39H2 TCF3 TCL1A
TRAF1 TYMS VDAC1 ZHX2
Housekeepers
ACTB DDX5 EEF1G EIF4A1
GAPDH PPIA PRKG1 RPL19
RPL4 RPL6 RPS29 TBP
Process Controls
ANT POS1
 

Ordering Information

The HTG EdgeSeq Lymphoma Panel contains all reagents and consumables required for use on the automated HTG EdgeSeq system. It also contains forward and reverse primers used to integrate the assay with Illumina MiSeq sequencing instruments.

Additional materials required, but not supplied by HTG, include general supplies (gloves, tips, razors, etc.), PCR reagents, bead-purification reagents, and sequencing library QC and quantification reagents.

Illumina-Compatible Configurations

Product # Configuration
Illumina
916-007-208 HTG EdgeSeq Lymphoma Panel (2x8)
916-007-008 HTG EdgeSeq Lymphoma Panel (4x8)
916-007-224 HTG EdgeSeq Lymphoma Panel (1x24)
916-007-024 HTG EdgeSeq Lymphoma Panel (4x24)
916-007-096 HTG EdgeSeq Lymphoma Panel (1x96)
Ion Torrent
916-007-308 HTG EdgeSeq Lymphoma Panel (2x8)
916-007-108 HTG EdgeSeq Lymphoma Panel (4x8)
916-007-324 HTG EdgeSeq Lymphoma Panel (1x24)
916-007-124 HTG EdgeSeq Lymphoma Panel (4x24)

Submit this form today to have an HTG representative contact you:

 

Last updated: 01/04/2017 - 07:37

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