HTG EdgeSeq Immuno-Oncology Assay

The sensitivity and dynamic range of Next-Generation Sequencing (NGS) paired with the specificity of HTG EdgeSeq chemistry enables measurement of the expression of hundreds of immuno-oncology genes with minimal sample input required.

The HTG EdgeSeq Immuno-Oncology Assay is comprised of probes targeting 549 genes implicated in the host immune response to tumors. The assay can be used to measure expression levels of drug targets, tumor infiltrate composition, and total immune cell composition using a single section of FFPE tissue.

Gain a deeper understanding of tumor immune response

  • One assay, many applications

    Characterizing immune response in tumors Profiling tumor infiltrating cells (TILs)
    Measuring total T-cell load Assessing tumor microenvironment
    Evaluating drug targets Evaluating prognostic drug signatures
    Monitoring patient response to drug treatment  
  • Just one FFPE section required | Conserve precious samples

  • Compatible with Illumina and Ion Torrent sequencing instruments | Get more out of your sequencer

  • Extraction-free chemistry | Eliminate biases introduced by RNA extraction

Automated on the HTG EdgeSeq system

Sample Input & Compatibility

Sample Type Recommendation

FFPE Tissue

A single 5 µm FFPE tissue section


500 µl

Extracted RNA

> 25 ng


Biopharma and HTG Molecular Diagnostics brochure
HTG EdgeSeq Immuno-Oncology brochure
HTG EdgeSeq Immuno-Oncology Gene List
HTG EdgeSeq Immuno-Oncology Gene List by Group
HTG EdgeSeq Immuno-Oncology sales sheet


Gene Expression Measurement of Immuno-Oncology Targets in a Single FFPE Section Using a Novel Targeted Sequencing Assay (presented at AACR 2016)

Ordering Information

The HTG EdgeSeq Immuno-Oncology Assay contains all reagents and consumables required for use on the automated HTG EdgeSeq system. It also contains forward and reverse primers used to integrate the assay with Illumina or Ion Torrent sequencing instruments.

Additional materials required, but not supplied by HTG, include general supplies (gloves, tips, razors, etc.), PCR reagents, bead-purification reagents, and sequencing library QC and quantification reagents.

Illumina - Compatible Configurations

Product #



HTG EdgeSeq Immuno-Oncology Assay (2x8)


HTG EdgeSeq Immuno-Oncology Assay (4x8)


HTG EdgeSeq Immuno-Oncology Assay (1x24)


HTG EdgeSeq Immuno-Oncology Assay (4x24)


HTG EdgeSeq Immuno-Oncology Assay (1x96)

Ion Torrent - Compatible Configurations

Product #



HTG EdgeSeq Immuno-Oncology Assay (2x8)


HTG EdgeSeq Immuno-Oncology Assay (4x8)


HTG EdgeSeq Immuno-Oncology Assay (1x24)


HTG EdgeSeq Immuno-Oncology Assay (4x24)

For Research Use Only. Not for use in diagnostic procedures.

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Last updated: 01/04/2017 - 07:36

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