DLBCL Cell of Origin Header
HTG EdgeSeq DLBCL Cell of Origin Assay

HTG EdgeSeq DLBCL Cell of Origin Assay

The HTG EdgeSeq DLBCL Cell of Origin Assay characterizes diffuse large B-cell lymphoma (DLBCL) into the activated B-cell like (ABC), germinal center B-cell like (GCB) or unclassified subtype using a single 5 µm formalin-fixed, paraffin-embedded (FFPE) tissue section including core needle biopsies. The DLBCL Cell of Origin Assay utilizes our automated HTG EdgeSeq system coupled with the sensitivity and dynamic range of next-generation sequencing (NGS)-based detection, and can be performed in your lab using the
HTG EdgeSeq system, in our VERI/O lab as a service, or at one of the Qualified Service Provider (QSP) sites.

For Research Use Only. Not for Use in Diagnostic Procedures.

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Features and Benefits

  • Simultaneous, quantitative detection of 92 transcripts associated with B-cell lymphomas
  • Classification and profiling from a single FFPE tissue section including needle core and other small biopsies 
  • Extraction-free: reduce extraction-associated data bias and sample loss
  • Gain insights faster: results in as little as 36 hours
  • Simplified data analysis: raw numerical data output provided in Excel format

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Sample Requirements

Sample Type HTG EdgeSeq DLBCL Cell of Origin Assay

Larger sample input amounts must be diluted. Please consult the assay package insert and HTG EdgeSeq System User Manual for more details. 

FFPE TissueOne 5 µm section

Performance

Benefit from NGS detection sensitivity and dynamic range to consistently measure small changes in high- and low-expressing genes.

Expression of PDCD1 and CD274 (PD-1 and PDL-1) is consistent across the titrations, indicating low expression genes are detected using the HTG EdgeSeq DLBCL Cell of Origin Assay in small amounts of FFPE tissue.

DLBCL ABC Graph

DLBCL GCB Graph

Applications

Recent advances in diffuse large B-cell lymphoma research have shown that determining whether the tumor is a "double-hit" or "double-expressor" tumor may be important. Unlike traditional methods, such as immunohistochemistry (IHC), the HTG EdgeSeq DLBCL Cell of Origin Assay enables researchers to consolidate the measurement of many important biomarkers into a single assay and workflow.

Sample GCB2 shows elevated expression of BCL2, MYC, and BCL6 indicative of a potential double/triple expressor profile.

Additional applications include: 

  • Assessment of potential of drug target candidates
  • Assessment of the additional lymphoma-associated genes for signature development 

Ordering Information

When placing an order, please specify the catalog number. The HTG EdgeSeq DLBCL Cell of Origin Assay is compatible with Illumina MiSeq sequencer. 

Kit Configurations for use with the Illumina MiSeq Sequencer

916-003-008 HTG EdgeSeq DLBCL Cell of Origin Assay (4 X 8)
916-003-024 HTG EdgeSeq DLBCL Cell of Origin Assay (4 X 24)
916-003-096 HTG EdgeSeq DLBCL Cell of Origin Assay (1 X 96)

For Research Use Only. Not for Use in Diagnostic Procedures.

Request A Quote

Resources and Publications

Learn more about the HTG EdgeSeq DLBCL Cell of Origin Assay

Resources

Biopharma and HTG Molecular Diagnostics Brochure
Download pdf 678KB

Comprehensive Tumor Profiling Brochure
Download pdf 464KB

HTG EdgeSeq DLBCL Cell of Origin Assay Gene List
Download pdf 341KB

Publications

Next-Generation Sequencing for DLBCL Classification
Download pdf 740KB


View All Publications

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For further information about the HTG EdgeSeq DLBCL Cell of Origin Assay, please fill out the registration form below or call us at (877) 507-3259.

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Page last updated January 18, 2018